Metabolism of testosterone by homogenates of rabbit liver and kidney.

The liver and kidneys were removed aseptically from non-fasted adult male rabbits, weighing 2.7 to 4.5 kilos, which were anesthetized with Dialurethane. The homogenate’ of the tissues was prepared by mixing the tissue, testosterone, and 50 ml. of the Ringer’s phosphate buffer in the Waring blendor for 2 minutes. The mixture was transferred to a Fernbath flask with another 50 ml. of buffer and incubated at 37” for 29 hours. The entire procedure was carried out with sterile precaution. The buffer and glassware were chilled in the refrigerator prior to use. The extraction and isolation procedures for the steroids were as previously described (2).